LOCUS       sequence-126162-         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), 
            pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM710
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    140..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac cgcggtggta
       61 caaagaaaat tcgacaaact gttatttttc tatctattta tttgaattgt gagcggataa
      121 caattacctt tgtcggcaat tgtgagcgga taacaattaa ataaagatat tctcgtcaaa
      181 caaatataaa taatataaac atggataaga aatactcaat aggcttagct atcggcacaa
      241 atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg
      301 ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg
      361 acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac
      421 gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag
      481 atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg
      541 aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa
      601 ctatctatca tctgcgaaa
//
