LOCUS       sequence-126258-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM731
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaaccgggtt ataatttgga tcaaactgca aagataagaa cttttagctt tatcttccaa
       61 actttatcgg aagttttttt gttcttatgg tcaagcagta ctgctgcagg gtgctaggat
      121 ttttttgggc ggccccgccc aaaaaaattg cagccgcatt aattcagtca cctcctagct
      181 gactcaaatc aatgcgtgtt tcataaagac cagtgatgga ttgatggata agagtggcat
      241 ctaaaacttc ttttgtagac gtatatcgtt tacgatcaat tgttgtatca aaatatttaa
      301 aagcagcggg agctccaaga ttcgtcaacg taaataaatg aataatattt tctgcttgtt
      361 cacgtattgg tttgtctcta tgtttgttat atgcactaag aactttatct aaattggcat
      421 ctgctaaaat aacacgctta gaaaattcac tgatttgctc aataatctca tctaaataat
      481 gcttatgctg ctccacaaac aattgttttt gttcgttatc ttctggacta cccttcaact
      541 tttcataatg actagctaaa tataaaaaat tcacatattt gcttggcaga gccagctcat
      601 ttcctttttg taattctccg gcactagcca gcatccgttt acgaccgttt tctaactcaa
      661 aaagactata tttaggtagt ttaatgatta agtctttttt aacttcctta tatcctttag
      721 cttctaaaaa gtcaatcgga tttttttcaa aggaacttct ttccataatt gtgatcccta
      781 gtaactcttt aacggatttt aacttcttcg atttccctt
//
