LOCUS       sequence-126262-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM732
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttctagctct aaaacggaac agggtggcgt aagctactgc aaagataaga acttttagct
       61 ttatcttcca aactttatcg gaagtttttt tgttcttatg gtcaagcagt actgctgcag
      121 ggtgctagga tttttttggg cggccccgcc caaaaaaatt gcagccgcat taattcagtc
      181 acctcctagc tgactcaaat caatgcgtgt ttcataaaga ccagtgatgg attgatggat
      241 aagagtggca tctaaaactt cttttgtaga cgtatatcgt ttacgatcaa ttgttgtatc
      301 aaaatattta aaagcagcgg gagctccaag attcgtcaac gtaaataaat gaataatatt
      361 ttctgcttgt tcacgtattg gtttgtctct atgtttgtta tatgcactaa gaactttatc
      421 taaattggca tctgctaaaa taacacgctt agaaaattca ctgatttgct caataatctc
      481 atctaaataa tgcttatgct gctccacaaa caattgtttt tgttcgttat cttctggact
      541 acccttcaac ttttcataat gactagctaa atataaaaaa ttcacatatt tgcttggcag
      601 agccagctca tttccttttt gtaattctcc ggcactagcc agcatccgtt tacgaccgtt
      661 ttctaactca aaaagactat atttaggtag tttaatgatt aagtcttttt taacttcctt
      721 atatccttta gcttctaaaa agtcaatcgg atttttttca aaggaacttc tttccataat
      781 tgtgatccct agtaactctt taacggattt taacttcttc gat
//
