LOCUS       sequence-126263-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C5C
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     94..156
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             169..186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             208..228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt
       61 cccaccatcg ggcgcggatc tcggtccgaa accatgaaat tcttagtcaa cgttgccctt
      121 gtttttatgg tcgtatacat ttcttacatc tatgcggccg ctccggaaca tcaccatcac
      181 catcacgatt acgatatccc aacgaccgaa aacctgtatt ttcagggcgc catggatatg
      241 tacgccccgc actcaatccg gatcgagggg gacgtcaccc tcggggggct gttccccgtg
      301 cacgccaagg gtcccagcgg agtgccctgc ggcgacatca agagggaaaa cgggatccac
      361 aggctggaag cgatgctcta cgccctggac cagatcaaca gtgatcccaa cctactgccc
      421 aacgtgacgc tgggcgcgcg gatcctggac acttgttcca gggacactta cgcgctcgaa
      481 cagtcgctta ctttcgtcca ggcgctcatc cagaaggaca cctccgacgt gcgctgcacc
      541 aacggcgaac cgccggtttt cgtcaagccg gagaaagtag ttggagtgat tggggcttcg
      601 gggagttcgg tctccatcat ggtagccaac atcctgaggc tcttccagat cccccagatt
      661 agttatgcat caacggcacc cgagctaagt gatgaccggc gctatgactt cttctctcgc
      721 gtggtgccac ccgattcctt ccaagcccag gccatggtag acattgtaaa ggccctaggc
      781 tggaattatg tgtctaccct cgcatcggaa ggaagttatg gagagaaagg tgtggagtcc
      841 ttcacgcaga tttccaaaga ggcaggtgga ctctcgattg cccagtccgt gagaatcccc
      901 caggaacgca aagacaggac cattgacttt gatagaatta tcaaacagct cctggacacc
      961 cccaactcca gggccgtcgt gatttttgcc aacgatgagg atataaagca gatccttgca
     1021 gcagccaaaa gagctgacca agttggccat tttctttggg tgggatcaga cagctgggga
     1081 tccaaaataa acccactgca c
//
