LOCUS       sequence-126359-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mTFP1 using KanMX6 as a selection marker in 
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: pFA6-mTFP1-KanMX6
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(91..288)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(294..1103)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1017..1036
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgcggccgca taggccacta gtggatctga tatcatcgat gaattcgagc tcgtttaaac
       61 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
      121 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
      181 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
      241 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgattagaaa
      301 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
      361 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
      421 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
      481 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
      541 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
      601 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
      661 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
      721 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
      781 aatacctgga atgctgtttt gccggggatc gcagtggtga gtaaccatgc atcatcagga
      841 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
      901 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
      961 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     1021 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgaa
     1081 acgtgagtct tttccttacc catggt
//
