LOCUS       sequence-126383-        1225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1_omega2R
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    43..67
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     187..206
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     400..422
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     414..431
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     415..431
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    432..488
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(501..517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(501..517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(514..536)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    525..541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(549..555,556..573,574..579))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    699..721
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 tgcttnctca tganatctca acnaacacat acaggcgact tagtttaccc gccaatatat
       61 cctgtcaaag cttggtctca agcggagtga gacgcacagc ttgtctgtaa gcggatgccg
      121 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
      181 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
      241 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
      301 gttgggaagg gcgatcggcg caggcctctt cgctattacg ccagctggcg aaagggggat
      361 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      421 cgacggccag tgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat
      481 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      541 attccacaca acatacgagc cggaagcata tagtgtaaag cctggggtgc ctaatgagtg
      601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      661 tgccagctgc gtctcacgct tgactgagac caagcttgtt acaccacaat atatcctgcc
      721 aagatctaat tccggggatc ggaaatccag aagcccgaga ggttgccgcc tttcgggctt
      781 tttctttttc aaaaaaaaaa atttataaaa cgatctgttg cggccggccg ccgggttgtg
      841 ggcaaaggcg ctcgacggtg ggcaaccgct tgcggttgtc cacgggcgga gccggtgcgc
      901 gtancgcatt gtccacaagc caagggcgac nataattgat atatatattc ataattgaaa
      961 agctaattga ncatactact ngctgtaact acttgccgna ncnaggggtg tttgcaanct
     1021 gttgatctga aanggctntn aacgtnctca cgtgcctttt nnatnacgat ttcccgtgac
     1081 cttntancga tttnccntta ctccnatanc gatttcccgt acctgatanc ganttcccng
     1141 gganantttn gaacgggccg aaancccngn aaaaaggntt tcgggnanna nngtttcngn
     1201 gaaaaaaccc tttccaaggt tcggg
//
