LOCUS       sequence-126406-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial 
            promoter. Cas genes are removed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY002 (pFnCpf1_min)
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(676..710)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatccac aggacgggtg
       61 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc
      121 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
      181 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
      241 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
      301 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
      361 aatttaactg tgataaacta ccgcattaaa gctagacaaa aaaatgacct tcataaatcg
      421 ctaatctaca acagtagaaa ttatttaaag ttcttagacc ggctactaat acaaaaggct
      481 ctagacatct acaacagtag aaattattta aagttcttag actgaattat ctgaaggcac
      541 aggaatagta gcatctacaa cagtagaaat tatttaaagt tcttagactt ttaacagtgg
      601 ccttattaaa tgacttctca tctacaacag tagaaattat ttaaagttct tagacccgtt
      661 tttgcctaaa tcagcgctag cattatacct aggactgagc tagctgtcaa ttagttattc
      721 ctattctgca cgaactcaaa atactcttca tttttgataa ccaaattgag ttt
//
