LOCUS       sequence-126413-         414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial 
            promoter. Cas genes are removed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY002 (pFnCpf1_min)
FEATURES             Location/Qualifiers
     source          1..414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(23..28,29..46,47..52)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttattaatca gataaaatat tttttacact ttatgcttcc ggctcgtatg ttaggaggtc
       61 tttatcatgt caatttatca agaatttgtt aataaatata gtttaagtaa aactctaaga
      121 tttgagttaa tcccacaggg taaaacactt gaaaacataa aagcaagagg tttgatttta
      181 gatgatgaga aaagagctaa agactacaaa aaggctaaac aaataattga taaatatcat
      241 cagtttttta tagaggagat attaagttcg gtttgtatta gcgaagattt attacaaaac
      301 tattctgatg tttattttaa acttaaaaag agtgatgatg ataatctaca aaaagatttt
      361 aaaagtgcaa aagatacgat aaagaaacaa atatctgaat atataaagga ctca
//
