LOCUS       sequence-126531-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hUCH37 (M148A/F149A) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pET151-hUCH37 (ISF1)(M148A/F149A)
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             59..100
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             101..121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaataatttt gtttaacttt aagaaggaga tatacatatg catcatcacc atcaccatgg
       61 taagcctatc cctaaccctc tcctcggtct cgattctacg gaaaacctgt attttcaggg
      121 aattgatccc ttcaccatga cgggcaatgc cggggagtgg tgcctcatgg aaagcgaccc
      181 cggggtcttc accgagctca ttaaaggatt cggttgccga ggagcccaag tagaagaaat
      241 atggagttta gagcctgaga attttgaaaa attaaagcca gttcatgggt taatttttct
      301 tttcaagtgg cagccaggag aagaaccagc aggctctgtg gttcaggact cccgacttga
      361 cacgatattt tttgctaagc aggtaattaa taatgcttgt gctactcaag ccatagtgag
      421 tgtgttactg aactgtaccc accaggatgt ccatttaggc gagacattat cagagtttaa
      481 agaattttca caaagttttg atgcagctat gaaaggcttg gcactgagca attcagatgt
      541 gattcgacaa gtacacaaca gtttcgccag acagcaagcg gctgaatttg atacgaagac
      601 atcagcaaaa gaagaagatg cttttcactt tgtcagttat gttcctgtta atgggagact
      661 gtatgaatta gatggattaa gagaaggacc gattgattta ggtgcatgca atcaagatga
      721 ttggatcagt gcagtaaggc ctgtcatag
//
