LOCUS       sequence-126972-        6168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF4F04
FEATURES             Location/Qualifiers
     source          1..6168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4595..4606
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgatgaaac gcactcgcgt agacgaggtg caatttggca cacgacccgt tcctcagacc
       61 tccggaggtg tgggcgttgg tgtcgtcggc gtaactggag gtgggccaac atccggcggc
      121 ggtggcactg ctactgtggg cgtcaatacg acgggcgtta ccattggtac cgtcgtgccc
      181 agtgctcata acgccaccat cagcgggatc ggttccattc accatcgcat ccttactcca
      241 cagcatggag gagcacaaac cattgcctat ttgccgtcca ccacgccgac ggctacgaat
      301 ttgaagacca caacctcgat tgtggatagc actaccgctg gaggaccagt aggagcagga
      361 gcacaggtgg ccgtaggagt tggctctgca gccggcggag gaggcgtggt ggtctccaca
      421 gggagcacgg gaacgcaaac attacagtat acaacatctt atagtgtggc ttcgatacag
      481 gctggtggaa ctctgaaagc caacacagcg gatggcgcga acacggtaca gattcatgtc
      541 acaggaggag gagctgcaaa caatcctgcc agtgcacaga ctgtttccag cagctcacaa
      601 acgggcacaa tccgtcagcg tacaatcagc ggcacccaga cagtggccac tgcagtgggt
      661 aatctagcca cgatttccca gcaacaaccg gttcaacaat cacctttggg caaggcccaa
      721 acgcctccat cgtccgtggt tgccaacagt atcccagtgg gtggaaccac cccaccacaa
      781 ggacagtcgg gaaatgccac gccacgctta aaagtggagg atgcgttgag ctacctggac
      841 caagtaaagt accagtatgc agaccagccg caaatctaca acaacttcct cgacataatg
      901 aaggaattca agtcgcactg catcgacaca cccggagtga ttgagcgagt ctccacactc
      961 ttcaaggggc acacagagct gatatacggc ttcaacatgt tcttaccgcc gggttacaag
     1021 atcgaaattc attcggatgc tcttggctgt tcggtgccgg tggtttcgat gccctcacca
     1081 ccgggagcgc caacgagcac aggaacggtg catatgctca ccggaaatag ttcgatgtcc
     1141 ggtgccggac atatcgccat caagacgacc aatgctgcga ctttaacacc ggcagcaggg
     1201 gctggagcag cggccgcagc agcagctgtg gctcagattc aatccgctgg agctgtaaat
     1261 ctaatgaccc acggcggagc ctcgcttacc cagaccacta ttcatgcatt gcaacaggcg
     1321 acgcctcctc aatcacaatc tccaggcgga ggtcatgtcc acgtgagtgt aacaaactct
     1381 acggcagcaa acgccgtagt gcccggacag ccaggcatct ccgtatcggc gcacaatgta
     1441 ccgcagaact actcgagaga tcgagaaagg gccaccataa cacctactgg gcaagtggct
     1501 ggagcagcgg caaacgtgaa tgcgtctgct agtatagttg tcggtggacc tccaacgccc
     1561 aattctctaa gtgagcttag ccctcatgga ggagcaggag gtgggccagg tgcgggagcg
     1621 gcgcagcaca atctgcatca catccagcag gcgcatcaat cgattttgct tggggagacg
     1681 ggacagcaga atcagccggt ggagttcaat cacgccataa cctatgtaaa taagatcaag
     1741 aatcgtttcc aaaaccagcc ggccaagtac aagaaattcc tcgaaattct gcacgattat
     1801 cagagggagc aaaaggtgat gaaggagggc agcctgaacc agggtaaaat gctcaccgag
     1861 caggaggtat acacgcaggt ggctaagctt tttggccagg atgaggattt gcttagagag
     1921 tttggccagt ttcttcctga tgccaccaac caccagtccg ggcagtacat gtccaagtca
     1981 gcgtctgtgc acaatgacca tggcaagcgt cccacggcta ctttaagcgg tggagctcac
     2041 ataaccatgt catctgcttc gccagcgcca agtggatccc ctctgcattt gggtgcaacg
     2101 actcttccgc agatcgacaa aagcgcccat gcagcggcca taggaaacct ttcggcggtg
     2161 aacactagtg tcagcattaa gacgtacaat aataatcagc aacagcagaa tcatgtgatc
     2221 ggttcagggc tgaatgcgac gcggaacgat attctctttg agaaggacta tcacgcgggt
     2281 ctgcagcagc aagcgcatca gcgaggagct ggagttggag gccatcatca tttggccggg
     2341 actgcagcgg gcgccaatat cggacggcct ggagtgggag ccagcgtgat ggtgtcgtac
     2401 gataaagagc atcgaaataa tcatcatgta caaaagtacg ttggccacgc gccaaaccag
     2461 aatctaacgc atggtcataa cgcaaagaaa tcaccaagtt atggcattcc ttcggtgatt
     2521 ggttccatgc cgcacatttc ggacaattcg ctcgaccgca gttcaccggg aattagttac
     2581 gccacgccac cattgccctc tggtccacat ggacagcata actctggttc agctacgagg
     2641 aggccgggtg atgacagttt agtcggacac tatgctagtg gagcgccgcc tgctaagcga
     2701 ccgaagcctt attgtcgtga tgtcagcttt tccgaagcat cgagcaagtg caccatctcc
     2761 gacgccgcgt tctttgacaa ggtgcgaaag gcgttgagaa gtccagaggt ctacgacaac
     2821 ttcctgcgat gcctgaccct ttttaaccag gaaattgtct ccaaaacgga acttcttggt
     2881 ctggtatcac cgttcttgat gaagttcccc gacctgctga gatggttcac cgactttctg
     2941 ggaccgccct ctggtcaacc tgctggagga ttgattgacg gcatgccctt agccgcaacg
     3001 caacgtcagg gaggtggaag cagtaatagt tcccatgatc gaggtaccag tcaccagagt
     3061 gccgccgagt atgtccagga tgtggatctg tcttcttgca agcggctggg tgcatcctat
     3121 tgtgctttgc cgcagtccac agtacctaag aagtgcagcg gacggacggc tctctgccgg
     3181 gaagtgctca acgacaaatg ggtatcgttc ccgacgtggg ccagcgagga ctccactttt
     3241 gttacatcgc gaaaaactca gttcgaggag acaatttaca gaacggagga cgagcgattc
     3301 gagctggacc tggtcatcga ggttaatagt gccacgattc gagtgctgga gaacttacag
     3361 aagaaaatgt cacgaatgtc caccgaggaa ttgagcaaat tccatctgga cgatcatctg
     3421 ggcggcacat ctcaaacgat acaccagcgg gccattcatc gcatctacgg cgacaagtcg
     3481 ggagagataa tcacgggaat gaagaaaaat ccgtttgtgg cggtgcctat tgtactgaag
     3541 cgactgaaag tcaaggagga ggagtggcga gaagcgcaaa agaccttcaa taagcaatgg
     3601 cgggaacaga acgagaagta ctacctcaag tctctcgatc accaggccat caactttaag
     3661 cccaacgaca tgaaagccct gcgctcaaag agtctgttta acgaaatcga gacgctgtac
     3721 gatgagcggc acgaccaaga ggacgacgcc atggaaccgt ttgggccgca tctggtgctg
     3781 ccgtacaagg ataaaaccat tctggacgat gccgccaacc tgctgattca tcatgtgaag
     3841 cgtcagactg gcatccagaa gcaggagaag caaaagatta agcagatcat ccgacaattt
     3901 gtgcctgacc ttttcttcgc gccgcgacag ccactaagcg atgacgaacg cgatgacgat
     3961 gacaatacga aaatggacgt ggactcgcca ctgggtcgca cggagtcttc gactcgaaat
     4021 gccaagagta cgcccagtga aagtgcctct ccggcgcgta gcaatgcaag cactagcagc
     4081 gtaacaccag cgggaattaa gaaagagact gatgactcga aagcgacgac aggatctttt
     4141 gcaccagcgt cctcggcaac ggcatcttca gcgacgcctg tggacgatgc tacgccatca
     4201 acatcctcag cagcggcggc agcatcagcg gcatcgtcat caactgtttc aggaaccgaa
     4261 ggcaagccaa aggatgaccc cctatcgtcg cacaaggaag aaggagccgg tagcaccagc
     4321 tctggagttg ccacgagtcc caggcaagcc caagacactg caggagcagg cgtcgatgtg
     4381 gaaatcaaac tggaacatcc tgcggacttt tccaatccaa agcttctacc gccgcacgcg
     4441 cacgggcaac gcgaagacga atcctacacg ctgttcttcg ctaacaacaa ttggtattta
     4501 ttcctgcggc tacatgcgat tctctgcgac cgtctgcatg ttatgtatga aagagcgcgt
     4561 ttgctggcca tcgaggagga gcgctgtagg gtgaatcgaa gggagagcac agccacggcg
     4621 ctgcggctaa agcccaaacc ggagattcaa gttgaagact actaccccac cttccttgat
     4681 atgctcaaga acgtcctaga cggcaacatg gactcgaata cgttcgagga cacgatgcgt
     4741 gaaatgtttg gcatttatgc ctacatctct ttcacgctgg ataaggttgt ctccaatgct
     4801 gttcgccagc tgcaatattg tgtcacagaa cgagccgcac tggattgtgt agagctgttt
     4861 gcaacggagc aaaggcgtgg ctgcaccgga ggattctgtc gagatgcgca caagacattt
     4921 gacagggaga tgtcgtatca gcggaaagct gagagcattc tcaacgagga gaattgcttc
     4981 aaagtgtaca tttacaaaat cgattgccgt gtaaccatag agctgcttga ttcggagccc
     5041 gaggaagtcg ataagccggc tgcgttgaag gcccagaagt tcagcaagta tgtagagcgg
     5101 ttggcgaatc ctgcgctcgg cggtggtggc aatactggcc gctccgattc ggcgctcggc
     5161 aatgacagcg ttgtagatgg gtccgatatc aagacggagg cggacgaaga tactgccgag
     5221 ttgcgctaca gagaaggtgg aatcggagga gcaggcaagg cgcgcttttt ggtgcgcaac
     5281 aagcggagat caaagctgct tgaggagcaa gtgcgtcagt tgtttgaaca taggcggaat
     5341 cggattgagc agcatggtac tgcggcctca cttgaaccca tctccgttga cagtgccatt
     5401 tcaagcaaca gcaccaccgg cggtggcggc agcggcacta ataacaacaa taataataat
     5461 aataataaca acagctgggg cggcaaacgt ctggagaggc tgggcgcccc acaggtgggc
     5521 ttggccgagc aatacgcctt taatgatcgc gacgagatca gcacaaatat cagcaacggg
     5581 cgatgttttg tgaccagcaa gaatctcaag ctacttaagt acgatgcagt gcgtcgtgcc
     5641 aagaagagtc attgccgcgt aactcaggcg aaatacgctc acttccaaac gtacgttaac
     5701 aagtggcttc agcaacatgt cagcgagcaa cagcagcaga actgcgtcga ctggctgctg
     5761 ggcaagacgg cagaccaaat caatgccagc ggctggggat ccgccagcaa aaccaagacg
     5821 gtacagcaga aggatacttc gaagacaccg tatcgcatct acaacagata cagagtggtg
     5881 cagagtgcag taagcggcgg tttgatgagt gccactgctc caactccaga tggttcagtt
     5941 ccggcggtca attccgcgcc atcaggctca gtttcaacgg tgaacaatgt acagggctcg
     6001 gcggcagtat cctcaacttc ccactcgatg tgcaacagta gcattttgtc cgcggactcc
     6061 accaccaata ccaatgcgtc agcacagcag cagcagcatt gcaacagtgc agcagctgca
     6121 acaacaccga atgcagaggc actgcaacag gtgcgaccca tggagagc
//
