LOCUS       sequence-127205-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a GFP circular RNA containing the antisense EMCV IRES in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Kramer MC, Liang D, Tatomer DC, Gold B, March ZM, Cherry S, Wilusz 
            JE
  TITLE     Combinatorial control of Drosophila circular RNA expression by 
            intronic repeats, hnRNPs, and SR proteins.
  JOURNAL   Genes Dev. 2015 Oct 8.
  PUBMED    26450910
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2015 Oct 8."
COMMENT     Sequence Label: pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisen...
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      121 ggtggaattc agtgacagtg gagattgtac agttttttcc tcgatttgtc aggatttttt
      181 ttttttgacg gagtttaact tcttgtctcc caggtaggaa gtgcagtggc gtaatctcgg
      241 ctcactacaa cctccacctc ctgggttcaa gcgtttctcc tgcctcagct ttccgagtag
      301 ctgggattac aggcgcctgc caccatgccc tgctgacttt tgtattttta gtagagacgg
      361 ggtttcacca tgttggccag gctggtcttg aactcctgac cgcaggcgat tggcctgcct
      421 cggcctccca aagtgctgag attacaggcg tgagccacca cccccggcct caggagcgtt
      481 ctgatagtgc ctcgatgtgc tgcctcctat aaagtgttag cagcacagat cactttttgt
      541 aaaggtacgt actaatgact ttttttttat acttcaggat ggcagcgtgc agctggccga
      601 ccactaccag cagaataccc ccatcggcga tggccctgtg ctgctgcccg ataaccacta
      661 cctgtccacc cagagcgccc tgtccaagga ccccaacgag aagcgcgatc acatgatcta
      721 cttcggcttc gtgaccgccg ccgccatcac ccacggcatg gacg
//
