LOCUS       sequence-127290-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 with His, MBP, and Strep tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     Gerard Cagney unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NM-01
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..69
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             115..132
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg ctagcatgac tggtggacag
       61 caaatgggtc gcggatccga attcgagctc cgtcgacaag cttgcggccg catgcatcac
      121 catcaccatc accccatgaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
      181 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
      241 gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc
      301 gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc
      361 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg
      421 gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg
      481 ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
      541 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
      601 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
      661 aagtacgaca ttaaagacgt ggggcgtgga taaacgctgg gcgcgaaagc gggtctgacc
      721 ttcctggttg accctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      781 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      841 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
      901 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt
//
