LOCUS       sequence-127292-         455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 with His, MBP, and Strep tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 455)
  TITLE     Gerard Cagney unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NM-01
FEATURES             Location/Qualifiers
     source          1..455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcatgcatca ccatcaccat caccccatga aaatcgaaga aggtaaactg gtaatctgga
       61 ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata
      121 ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc ccacaggttg
      181 cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt ggtggctacg
      241 ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc
      301 cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg
      361 aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag
      421 agatcccggc gctggataaa gaactgaaag cgaaa
//
