LOCUS       sequence-127335-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episomal plasmid encoding dCas9VP192.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: pCXLE-dCas9VP192-T2A-EGFP
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..69
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             106..216
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             268..378
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             430..540
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacagccc caagaagaag
       61 agaaaggtgg aggccagcct cgagtgcgaa agcggcgggc cggccgacgc ccttgacgat
      121 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
      181 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtcactacc cagcgctagc
      241 gtcgagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
      301 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
      361 tttgaccttg acatgctccc cgggtcacta cccagcgcta gcgtcgagtg cgaaagcggc
      421 gggccggccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
      481 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
      541 cccgggtcac tacccagcgc tagcgtcgag tgcgaaagcg gcgggccggc cgacgccctt
      601 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttga
//
