LOCUS       sequence-127386-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses Syt1-SNARE37aa-linker complex with p045.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Zhou Q, Lai Y, Bacaj T, Zhao M, Lyubimov AY, Uervirojnangkoorn M, 
            Zeldin OB, Brewster AS, Sauter NK, Cohen AE, Soltis SM, Alonso-Mori 
            R, Chollet M, Lemke HT, Pfuetzner RA, Choi UB, Weis WI, Diao J, 
            Sudhof TC, Brunger AT
  TITLE     Architecture of the synaptotagmin-SNARE machinery for neuronal 
            exocytosis.
  JOURNAL   Nature. 2015 Sep 3;525(7567):62-7. doi: 10.1038/nature14975. Epub 
            2015 Aug 17.
  PUBMED    26280336
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14975"; journalName: "Nature"; date: "2015-09-3- 3"; 
            volume: "525"; issue: "7567"; pages: "62-7"
COMMENT     Sequence Label: pDC184
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(73..120)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     116..134
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(172..216)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    475..499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(500..518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(785..805)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(806..835)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    894..918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(919..937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc
       61 ctgaggtttc agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag
      121 ttattgctca gcggtggcag cagcctaggt taattaagct gcgctagtag acgagtccat
      181 gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagtt acttgacggc
      241 cttcttggtg tcagacacgg ccctctccac gtagtccaca gcgtgttcca cattgtactc
      301 gatcctgtca atcatctccc cctggctctc caccagcatg gccatgtcca tgaacatatc
      361 gtgtagctcc cggatgctgt tctccaactt gatgatctca ctgtgcctgg tctcgatctc
      421 actgagggcc atatgtatat ctccttctta tacttaacta atatactaag atggggaatt
      481 gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgattatg cggccgtgta
      541 caatacgatt actttctgtt cgacttaagc attatgcggc cgcaagcttg tcgagctacc
      601 agtatttgcg cttgagcttg gctgcacttg tttcaaactg ggaggcccct gcctggaggg
      661 catctgcgcg atcatccagt tccgatagct tctggtctcg ctccaggacc ttgtccacat
      721 tcaccctcat gatgtccacc acctcatcca cctgggcctg ggtctgctgc agtctcctgt
      781 tgctgccctg aaaatacagg ttttcgtgat ggtgatggtg atggtgatgg tgatggtagt
      841 acgatgccat ggtatatctc cttattaaag ttaaacaaaa ttatttctac aggggaattg
      901 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcctaatgca ggagtcgcat
      961 aagggagagc gtcgagatcc cgg
//
