LOCUS       sequence-127420-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on 
            the C-terminus. CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: Cx43-sfGFP
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(7..720,721..723,724..726))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(52..73)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     660..681
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      723..732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccgctttac ttgtacagct cgtccatgcc gtgagtgatc ccggcggcgg tcacgaactc
       61 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcacgg actgggtgct
      121 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      181 gtggtcggcg agctgcacgc tgccgtcctc cacgttgtgg cggatcttga agttggcctt
      241 gatgccgttc ttctgcttgt cggcggtgat atagacgttg tggctgttga agttgtactc
      301 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      361 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagg tgccgtcgtc
      421 cttgaagctg atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
      481 gtggcgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
      541 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
      601 cttgccgttg gtggcatcgc cctcgccctc gccgcgcacg ctgaacttgt ggccgtttac
      661 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
      721 caccatggtg gcgaccggtg gatcccgggc ggaaatctcc aggtcatcag gccgaggcct
      781 gctgctggcg cggctgctgg ctctgctgga aggtcgttgg tccacgatgg ctaatggctg
      841 gagttcatgt ccagcagcaa cttttttggc attctggttg tcgtcgggga aatcgaacgg
      901 ctgggcgtgc gagttggaga tggtgcttcc ggcctgcccc atgcgatttt g
//
