LOCUS       sequence-127512-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the 
            N-terminus of the Connexin linking to msfGFP (FP fused to NT of 
            connexin). CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Stout RF Jr, Snapp EL, Spray DC
  TITLE     Connexin Type and Fluorescent Protein-fusion Tag Determine 
            Structural Stability of Gap Junction Plaques.
  JOURNAL   J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979.
  PUBMED    26265468
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 11. pii: jbc.M115.659979."
COMMENT     Sequence Label: msfGFP-Cx43
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(54..56,57..59,60..770)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(99..120)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     707..728
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      121 taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc
      181 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      241 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcgccacg
      301 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc agcttcaagg
      361 acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      421 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      481 agtacaactt caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
      541 aggccaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc gccgaccact
      601 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      661 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      721 agttcgtgac cgccgccggg atcactcacg gcatggacga gctgtacaag tccggactca
      781 gatctcgagc tcagatgggt gactggagtg ccttggggaa gcttctggac aaggtccaag
      841 cctactccac cgctggaggg aaggtgtggc tgtcagtgct cttcatattc agaatcctgc
      901 tcctggggac agctgttgag tcagcttggg gtgatgaaca gtctgccttt cgctgtaaca
      961 ctcaacaacc tggctgcgaa aacgtctgct atgacaagtc cttccccatc tctcacgtgc
     1021 gcttctgggt ccttcagatc atattcgtgt ctgtgcccac cctcctgtac ttggcccatg
     1081 tgttctatgt gatgagg
//
