LOCUS       sequence-127517-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N Trans V2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-288
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..45
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             46..66
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagcaatccg gagtatacat ggactacaag gacgacgatg acaagcccaa gaaaaagcgc
       61 aaggtctcta gacccgggga gcgccccttc cagtgtcgca tttgcatgcg gaacttttcg
      121 gataaaacta aattgagagt tcatacccgt actcataccg gtgaaaaacc gtttcagtgt
      181 cggatctgta tgcgaaattt ctccgttaga cataatttga ctagacatct acgtacgcac
      241 accggcgaga agccattcca atgccgaata tgcatgcgca acttcagtca atctacttct
      301 ttgcaaagac acctaaaaac ccacctgaga ggattcggcg gagtgctgga gaagggctgc
      361 tttgccaagg gtaccaatgt tttaatggcg gatgggtcta ttgaatgtat tgaaaacatt
      421 gaggttggta ataaggtcat gggtaaagat ggcagacctc gtgaggtaat taaattgccc
      481 agaggaagag aaactatgta cagcgtcgtg cagaaaagtc agcacagagc ccacaaaagt
      541 gactcaagtc gtgaagtgcc agaattactc aagtttacgt gtaatgcgac ccatgagttg
      601 gttgttagaa cacctcgtag tgtccgccgt ttgtctcgta ccattaaggg tgtcgaatat
      661 tttgaagtta ttacttttga gatgggccaa aagaaagccc ccgacggtag aattgttgag
      721 cttgtcaagg aagtttcaaa gagctaccca atatctgagg ggcctgagag agccaacgaa
      781 ttagtagaat cctatagaaa ggcttcaaat aaagcttatt ttgagtggac tattgagg
//
