LOCUS       sequence-127601-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TRC lentiviral vector for shRNA against mouse Dnd1 #7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLKO.1-shDnd1-No7
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    137..254
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     732..750
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 gattcttggc tttatatatc ttgtggaagg acgaaacacc ggcctctatg agttccgcct
       61 aatctcgaga ttaggcggaa ctcatagagg tttttgaatt ctcgacctcg agacaaatgg
      121 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      181 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      241 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
      301 agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
      361 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
      421 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
      481 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
      541 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
      601 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
      661 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
      721 ctgcccgcgc ggtgttccgc attctgcaag
//
