LOCUS       sequence-127610-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. Cre-removable and tet/dox controllable 
            expression of FLAG-HA-tagged transgene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLVE-FH-IN
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(200..219)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     810..829
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
       61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
      121 acacgatgat aatatggcca caaccatgat tgaacaagat ggattgcacg caggttctcc
      181 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      241 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      301 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
      361 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      421 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      481 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      541 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      601 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      661 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
      721 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
      781 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ct
//
