LOCUS       sequence-127611-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. Cre-removable and tet/dox controllable 
            expression of FLAG-HA-tagged transgene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLVE-FH-IN
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    54..171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      197..206
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             206..229
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             230..256
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(482..499)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     709..728
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tcgtgaggaa tttcgacatt taaatttaat taatctcgac ggtatcggtt aacttttaaa
       61 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      121 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac
      181 gagactagcc tcgagggcca ccatggacta caaggacgac gatgacaagt acccttatga
      241 cgtgcccgat tacgcttctg gtggcggtgg ctcgggcgga ggtgggtcgg gtggcggcgg
      301 atcaggatcc taaccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
      361 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
      421 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
      481 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
      541 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
      601 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
      661 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
      721 agcgtattca acaagggg
//
