LOCUS       sequence-127650-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2). ApR, expression vector with 
            rrnBT1T2-Pm-MCSII, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 51
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(172..630)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(309..330)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     524..543
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattcca agctccaggg ggatcggtta gaaattgtaa
      181 acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
      241 aataggccga aatcggcaaa atcccttata aatcaaaaga atagcccgag atagggttga
      301 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
      361 ggcgaaaaac cgtctatcag ggcgatggcc gcccactacg tgaaccatca cccaaatcaa
      421 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
      481 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
      541 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
      601 ccgcgcttaa tgcgccgcta cagggcgcgt actatggttg ctttgacgag cacgtataac
      661 gtgctttcct cgttagaatc agagcgggag ctaaacagga ggccgattaa agggatttta
      721 gacaggaacg gtacgccagc aagctgggcg gccgcaagct tgtcgacgga tccgagctcg
      781 gtaccatgga tatctgcagt tcttgccata tgcccgggtg aaccgaattg ggaattcagg
      841 cattgacgaa ggcattcagc tcaccatctt tgttgcgtgt gatgaatatc ggctgccggc
      901 ccatctgcgt ggtgtaatag tcgttcttct cgggaatctg gctctcgtgg gcgagataaa
      961 tccagttgcc ctcaaagatg tgtttcatct ctaaatcgaa cagccgaggg tcggtgaaca
     1021 tctcgcgctt gcagcggtag atgccctcgt tctcatcttc ttcaacgaga ctatctatat
     1081 agtcga
//
