LOCUS       sequence-127659-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with 
            an N-terminal EGFP tag and C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-S305E-3xFLAG
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     152..173
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
       61 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      121 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      181 ccgggatcac tctcggcatg gacgagctgt acaagtactc agatctcgag ctcaagcttg
      241 gtaccatggc gacctgcatc ggggagaaga tcgaggattt taaagttgga aatctgcttg
      301 gtaaaggatc atttgctggt gtctacagag ctgagtccat tcacactggt ttggaagttg
      361 caatcaaaat gatagataag aaagccatgt acaaagcagg aatggtacag agagtccaaa
      421 atgaggtgaa aatacattgc caattgaaac atccttctat cttggagctt tataactatt
      481 ttgaagatag caattatgtg tatctggtat tagaaatgtg ccataatgga gaaatgaaca
      541 ggtatctaaa gaatagagtg aaacccttct cagaaaatga agctcgacac ttcatgcacc
      601 agatcatcac agggatgttg tatcttcatt ctcatggtat actacaccgg gacctcacac
      661 tttctaacct cctactgact cgtaatatga acatcaagat tgctgatttt gggctggcaa
      721 ctcaactgaa aatgccacat gaaaagcact atacattatg tggaactcct aactacattt
      781 caccagaaat tgccactcga agtgcacatg gccttgaatc tgatgtttgg tccctgggct
      841 gtatgtt
//
