LOCUS       sequence-127662-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and 
            S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 
            3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet 
            P, Ramaekers FC, Bornens M, Grand-Perret T
  TITLE     Autophosphorylation of polo-like kinase 4 and its role in centriole 
            duplication.
  JOURNAL   Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 
            10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
  PUBMED    20032307
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-06-0505"; journalName: "Mol Biol Cell"; date: 
            "2010-02-15- 15"; volume: "21"; issue: "4"; pages: "547-61"
COMMENT     Sequence Label: pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     151..172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
       61 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      121 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      181 cgggatcact ctcggcatgg acgagctgta caagtactca gatctcgagc tcaagcttgg
      241 taccatggcg acctgcatcg gggagaagat cgaggatttt aaagttggaa atctgcttgg
      301 taaaggatca tttgctggtg tctacagagc tgagtccatt cacactggtt tggaagttgc
      361 aatcatgatg atagataaga aagccatgta caaagcagga atggtacaga gagtccaaaa
      421 tgaggtgaaa atacattgcc aattgaaaca tccttctatc ttggagcttt ataactattt
      481 tgaagatagc aattatgtgt atctggtatt agaaatgtgc cataatggag aaatgaacag
      541 gtatctaaag aatagagtga aacccttctc agaaaatgaa gctcgacact tcatgcacca
      601 gatcatcaca gggatgttgt atcttcattc tcatggtata ctacaccggg acctcacact
      661 ttctaacctc ctactgactc gtaatatgaa catcaagatt gctgattttg ggctggcaac
      721 tcaactgaaa atgccacatg aaaagcacta tacattatgt ggaactccta actacatttc
      781 accagaaatt gccactcgaa gtgcacatgg ccttgaatct gatgtttggt ccctgggctg
      841 tatgttttat acattactta tcgggagacc acccttcgac actgacacag tcaagaacac
      901 attaaataaa gtagtattgg cagattatga aatgccatct tt
//
