LOCUS       sequence-127663-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of hSERT with N-terminal YFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Sucic S, Dallinger S, Zdrazil B, Weissensteiner R, Jorgensen TN, 
            Holy M, Kudlacek O, Seidel S, Cha JH, Gether U, Newman AH, Ecker GF,
            Freissmuth M, Sitte HH
  TITLE     The N terminus of monoamine transporters is a lever required for the
            action of amphetamines.
  JOURNAL   J Biol Chem. 2010 Apr 2;285(14):10924-38. doi: 
            10.1074/jbc.M109.083154. Epub 2010 Jan 29.
  PUBMED    20118234
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.083154"; journalName: "J Biol Chem"; date: 
            "2010-04-2- 2"; volume: "285"; issue: "14"; pages: "10924-38"
COMMENT     Sequence Label: pEYFP-C1-hSERT
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(54..56,57..59,60..770)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(99..120)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(360..379)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     707..728
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      121 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      181 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      241 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      301 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      361 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      421 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      481 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      541 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      601 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      661 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      721 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
      781 gatctcgagc catggagacg acgcccttga attctcagaa gcagctatca gcgtgtgaag
      841 atggagaaga ttgtcaggaa aacggagttc tacagaaggt tgttcccacc ccaggggaca
      901 aagtggagtc cgggcaaata tccaatgggt actcagcagt tccaagtcct ggtgcgggag
      961 atgacacacg gcactctatc ccagcgacca ccaccaccct agtggctgag cttcatcaag
     1021 gggaacggga gacctggggc aagaaggtgg atttccttct ctcagtgatt ggct
//
