LOCUS       sequence-127669-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mouse vinculin with GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Leerberg JM, Gomez GA, Verma S, Moussa EJ, Wu SK, Priya R, Hoffman 
            BD, Grashoff C, Schwartz MA, Yap AS
  TITLE     Tension-sensitive actin assembly supports contractility at the 
            epithelial zonula adherens.
  JOURNAL   Curr Biol. 2014 Aug 4;24(15):1689-99. doi: 
            10.1016/j.cub.2014.06.028. Epub 2014 Jul 24.
  PUBMED    25065757
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.06.028"; journalName: "Curr Biol"; date: 
            "2014-08-4- 4"; volume: "24"; issue: "15"; pages: "1689-99"
COMMENT     Sequence Label: GFP-mouse vinculin full length (889)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(50..52,53..55,56..766)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(50..52,53..55,56..766)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(95..116)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(356..375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     703..724
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa
       61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      181 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      241 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      481 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      541 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      661 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc
      781 tcgagctcaa gcttcgaatt ccgccaccat gccggtgttt catacgcgta cgatcgagag
      841 catcctggag ccggtggcgc
//
