LOCUS       sequence-127734-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2226
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..215
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(816..981)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     902..926
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
       61 gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
      121 gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag
      181 tgaagttcct attctctaga aagtatagga acttctgaag tggggattta aatgcggccg
      241 cgctgagggt ttaatcccaa agctaagagt cccattttat tcttctatat gtatattttc
      301 gatactctaa accaccctac aatgtagccc tatactaaat ctgctcaatt ttcagcttct
      361 acaagtgact cgaggccacg tggaaagatc caactactcc agcacaacga ttcaatataa
      421 tcgattgctc cactcataag aggcaagaac aagcttcaac ttttggtaag ccgccgttta
      481 taaacaggga agatgtcctt tgtcaaggga ggcacagagc atggccaatt tggcaaattg
      541 caggtttttc tgagtgaaaa atgaaaaagc attgtagtag agtcggctca ctgaaaaacc
      601 ggggaggacg aaaaggtttc cagccacagt tgtagtcacg tgcgcgccat gctgactaat
      661 ggcagccgtc gttgggcaga agagaattag tatggtacag gatacgctaa ttgcgctcca
      721 actaccaagg ttgttgaggg aacactgggg caataggctg tcgccattca agagcagatt
      781 aagtcctcag cgagctcgca tggaatgcgt gcgatgagcg acctcatgct atacctgaga
      841 aagcaacctg acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta
      901 agagtcactt taaaatttgt atacacttat tttttttata acttatttaa taataaaaat
      961 cataaatcat aagaaattcg cttattta
//
