LOCUS       sequence-127736-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2226
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..90
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     74..90
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    560..593
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tgccaggcat caaactaagc agaaggcccc tgacggatgg cctttttgcg tttctacaaa
       61 ctctttctgt gttgtaaaac gacggccagt cttaagctcg ggccccctgg gcggttctga
      121 taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg tttttttatg
      181 gggggagttt agggaaagag catttgtcag aatatttaag ggcgcctgtc actttgcttg
      241 atatatgaga attatttaac cttataaatg agaaaaaagc aacgcacttt aaataagata
      301 cgttgctttt tcgattgatg aacacctata attaaactat tcatctatta tttatgattt
      361 tttgtatata caatatttct agtttgttaa agagaattaa gaaaataaat ctcgaaaata
      421 ataaagggaa aatcagtttt tgatatcaaa attatacatg tcaacgataa tacaaaatat
      481 aatacaaact ataagatgtt atcagtattt attatgcatt tagaataaat tttgtgtcgc
      541 ccttattcga ctcactatag aagttcctat tctctagaaa gtataggaac ttcacttcat
      601 tttatttaaa tttgcggccg cgctgaggtc ttaatctggt gaggatttac ggtatgatca
      661 tgctggtaag cttcgttact catgctttgg ttggcttctt gaaagtgtct agttcacttt
      721 atgcccggtt ataaacataa actttcacag ggttctcttt gatcacctct ttgtttaatc
      781 ttatgaatcc aagggatttt atcggtcaaa ggggaaatca atgcgaaaga cagtaatgat
      841 gagagaaaaa ctctccgtaa ccaccaagtt tggttcagcg cgacgagatt tttatcgatt
      901 atcgagaaaa atacctgtat atctacattt ctatgtcagt gatatatact tcttagata
//
