LOCUS       sequence-127804-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2198
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      77..266
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    314..347
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        402..745
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aaatcataag aaattcgctt atttagaagt gtcaacaacg tatctaccaa cggaatgcgt
       61 gcgatcgcgt gcattcatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct
      121 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa
      181 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc
      241 ttgagaaggt tttgggacgc tcgaagatcg cgtcagctga agcttcgtac gctgcaggtc
      301 gacaaccctt aatataactt cgtataatgt atgctatacg aagttattag gtctagagat
      361 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
      421 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
      481 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
      541 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca
      601 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
      661 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
      721 acatcacatc cgaacataaa caaccatggg taaaaagcct gaactcaccg cgacgtctgt
      781 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
      841 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
      901 tagctg
//
