LOCUS       sequence-127937-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2228
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    154..187
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga gtagggaact
       61 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg cccgggctaa
      121 ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta gaaagtatag
      181 gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgta tccggctgtt
      241 ccttcatagc cctttcaatg aacgttgcag ccctttgaag attggccatt ttgtcaggac
      301 tcgagcctga cagttggacc aacgcaactt taattttttg tgaaagaatc ttcgaagcac
      361 tcatactggc gatcttcacg ccctcctgct attacaaaag ctgtgttttt acaagaatca
      421 aattaagtta gcaagatatt atacaacatt attgataatt tcaatatcgt gttcgtacct
      481 gatgacgtat ctgtgcattg ataaggcccg catggtttca gaaagcagag cggaacgatt
      541 ccaaattagt ggccttgtgc tttgcatgtc aattgtgtta ccttcagctc gtggatttgt
      601 tttatcaata cacagtctac agtcaagaat ttttttttat caaattttgc gttcgagcgt
      661 ataaaatagc cgctgtagct acttaagttc ctgttcagcg atagtttttt tccatcacac
      721 gtactatggc aattaagtcc tcagcgagct cgcatggaat gcgtgcgatg agcgacctca
      781 tgctatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa taagaatttt
      841 cgttttaaaa cctaagagtc actttaaaat ttgg
//
