LOCUS       sequence-127980-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CDS of the Blue fluorescente protein as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pBFP (GB0025)
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..755
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(858..876)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(859..876)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(894..910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(894..910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgaatgagcg
       61 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
      121 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
      181 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
      241 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
      301 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
      361 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
      421 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
      481 tcaccgaaac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
      541 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
      601 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
      661 aggaggccaa caacgaaacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
      721 acctccctag caaactgggg cacaagctta attgagcttc gagaccactc atcgcccaat
      781 cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg
      841 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
      901 ctgtttcctg tgtgaaa
//
