LOCUS       sequence-127992-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for 
            noninvasive measurements of intrabacterial pH in live cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Vandal OH, Pierini LM, Schnappinger D, Nathan CF, Ehrt S
  TITLE     A membrane protein preserves intrabacterial pH in intraphagosomal 
            Mycobacterium tuberculosis.
  JOURNAL   Nat Med. 2008 Aug;14(8):849-54. doi: 10.1038/nm.1795. Epub 2008 Jul 
            20.
  PUBMED    18641659
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm.1795"; 
            journalName: "Nat Med"; date: "2008-08"; volume: "14"; issue: "8"; 
            pages: "849-54"
COMMENT     Sequence Label: pUV15-pHGFP
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(389..416)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(506..523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actccagtga aaagttcttc tcctttactc ataccggtgc ctccttaatt aagcatgcgg
       61 atcgtgctca tttcgggcgg cgaatctctc ggcgtcgaaa tgatgtatgc cgtgctggtc
      121 gtacacacgg gtactcccag agcctactcg accgagcaca cgcaggacaa actcgcccac
      181 cccgggtggg ttctcccgct cgtcagagac ccttctcaca ccgggcctgt gcgtgcgaac
      241 gcacagatca acgccgggat ggcgtagagc tcactgcgca gcaccgtggg gatgcggccg
      301 atcatcacgt cgcggatcgt gccgcctccc acggccgtga cggtgccgac gatccaatat
      361 ttctagagtt taaacagtat taaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      421 agctaatcaa ctagttctag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      481 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      661 ccgcgttgct ggcgtttttc c
//
