LOCUS       sequence-128002-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated
            STIM1 protein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Kyung T, Lee S, Kim JE, Cho T, Park H, Jeong YM, Kim D, Shin A, Kim 
            S, Baek J, Kim J, Kim NY, Woo D, Chae S, Kim CH, Shin HS, Han YM, 
            Kim D, Heo WD
  TITLE     Optogenetic control of endogenous Ca channels in vivo.
  JOURNAL   Nat Biotechnol. 2015 Sep 14. doi: 10.1038/nbt.3350.
  PUBMED    26368050
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Sep 14. doi: 10.1038/nbt.3350."
COMMENT     Sequence Label: pCMV-OptoSTIM1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(43..45,46..48,49..759)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(43..45,46..48,49..759)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(88..109)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(349..368)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     696..717
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcca ccatggtgag caagggcgag
       61 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      121 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      181 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      241 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      301 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      361 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      421 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      481 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      541 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      601 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      661 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      721 gccgccggga tcactctcgg catggacgag ctgtacaagc caccggtcgc caccatgaag
      781 atggacaaaa agaccatcgt ctggtttcgg agagatttga gaatagaaga taatcccgcg
      841 ctcgccgccg cggcccacga gggttccgtc ttccccgttt tcatttggtg tcctgaagaa
//
