LOCUS       sequence-128015-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor reporting on endogenous Cdc42 activation (for 
            Lentivirus production).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O
  TITLE     Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation 
            during prototypical edge protrusion and retraction dynamics.
  JOURNAL   Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.
  PUBMED    26912264
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:21901. doi"; pages: " 
            10.1038/srep21901"
COMMENT     Sequence Label: pLenti-Cdc42-2G
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             136..165
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccgact ctagaggatc cggagatata ccatggcaca ccatcaccac
      121 catcacggga gcggggagca gaaactaata tcagaggagg atcttggctc aggatcctcc
      181 accgacggca tggacgagct gtacaagggt gctagcggag gaatggtgag caagggcgag
      241 gagaccacaa tgggcgtaat caagcccgac atgaagatca agctgaagat ggagggcaac
      301 gtgaatggcc acgccttcgt gatcgagggc gagggcgagg gcaagcccta cgacggcacc
      361 aacaccatca acctggaggt gaaggaggga gcccccctgc ccttctccta cgacattctg
      421 accaccgcgt tcgcctacgg caacagggcc ttcaccaagt accccgacga catccccaac
      481 tacttcaagc agtccttccc cgagggctac tcttgggagc gcaccatgac cttcgaggac
      541 aagggcatcg tgaaggtgaa gtccgacatc tccatggagg aggactcctt catctacgag
      601 atacacctca agggcgagaa cttccccccc aacggccccg tgatgcagaa gaagaccacc
      661 ggctgggacg cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc
      721 aagcacaagc tgctgctgga gggcggcggc caccaccgcg ttgacttcaa gaccatctac
      781 agggccaaga aggcggtgaa gctgcccgac tatcactttg tggaccaccg catcgagatc
      841 ctgaaccacg acaaggacta caacaaggtg accgtttacg agagcgccgt ggcccgcaac
      901 tccggagaca tccagaaccc tgacatcacg agttcacgat accgtgggct cccagcacct
      961 ggacctagcc cagctgataa gaaacgctca gggaagaa
//
