LOCUS       sequence-128018-         273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for amiR-eGFP knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pAWK
FEATURES             Location/Qualifiers
     source          1..273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           52..93
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           100..126
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           190..231
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 ggtcagcttg gttttggcca ctgactgacc aagctgactg aagttcatca ggacacaagg
       61 cctgttacta gcactcacat ggaacaaatg gcccagatcc tggaggcttg ctgaaggctg
      121 tatgctgtgt agttgtactc cagcttgtgt tttggccact gactgacaca agctggtaca
      181 actacacagg acacaaggcc tgttactagc actcacatgg aacaaatggc ccagatctgg
      241 ccgcactcga gatatctaga cccagctttc ttg
//
