LOCUS       sequence-128170-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Wang H, Boisvert D, Kim KK, Kim R, Kim SH
  TITLE     Crystal structure of a fibrillarin homologue from Methanococcus 
            jannaschii, a hyperthermophile, at 1.6 A resolution.
  JOURNAL   EMBO J. 2000 Feb 1. 19(3):317-23.
  PUBMED    10654930
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2000 Feb 1. 19(3):317-23."
COMMENT     Sequence Label: pET21a.MJ0697
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             886..903
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atggaagaca ttaaaatcaa agagattttt gaaaacatct
      181 atgaagttga tttaggagat ggcttaaaaa gaatagcaac aaaatctatt gttaagggaa
      241 aaaaagtata tgatgaaaaa ataataaaaa ttggagatga agagtataga atttggaatc
      301 caaataaaag taagttggca gctgcaataa ttaaaggttt aaaggttatg ccaataaaaa
      361 gagattcaaa aatcttatac ttaggagctt cagctggaac tacaccatct cacgttgcag
      421 atattgctga taaaggcatt gtatatgcca tagagtatgc accaaggatt atgagagagc
      481 ttttagatgc atgtgctgag agggaaaaca taatcccaat tttaggagat gcaaataaac
      541 ctcaagaata tgcaaatatt gttgaaaagg tagatgttat ctatgaggat gttgctcagc
      601 caaatcaagc ggagatttta attaaaaatg ctaagtggtt tttaaagaaa ggcggttatg
      661 gaatgatagc aataaaggca aggagtatag atgttacaaa agacccaaaa gagattttta
      721 aagaacaaaa agaaatttta gaggcaggag gttttaaaat agttgatgag gtagatattg
      781 agccgtttga aaaagaccac gtcacgtttg taggtatttg ggagggaaaa tgactcgagg
      841 gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac
      901 cactgagatc cggctgcta
//
