LOCUS       sequence-128186-        1500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a STIM1_1-250 fragment mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma G, Wei M, He L, Liu C, Wu B, Zhang SL, Jing J, Liang X, Senes A, 
            Tan P, Li S, Sun A, Bi Y, Zhong L, Si H, Shen Y, Li M, Lee MS, Zhou 
            W, Wang J, Wang Y, Zhou Y
  TITLE     Inside-out Ca(2+) signalling prompted by STIM1 conformational 
            switch.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7826. doi: 10.1038/ncomms8826.
  PUBMED    26184105
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7826. doi"; pages: " 
            10.1038/ncomms8826"
COMMENT     Sequence Label: mSTIM1_1-250-CFP
FEATURES             Location/Qualifiers
     source          1..1500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      775..784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(781..783,784..786,787..1500)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(826..847)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1087..1106)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1434..1455
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggatgtgt gcgcccgtct tgccctgtgg cttctttggg ggctccttct gcatcagggc
       61 cagagtctca gccatagtca cagtgaaaag aatacaggag ctagctccgg ggcgacttct
      121 gaagagtcta ccgaagcaga gttttgccga attgacaagc ccctgtgcca cagtgaggat
      181 gagaagctca gctttgaggc cgtccgaaac atccataagc tgatggatga cgatgccaat
      241 ggtgatgtgg atgtggaaga aagtgatgag ttcctaaggg aagacctcaa ttaccatgac
      301 ccaacagtga aacatagcac cttccatggt gaggataagc ttatcagcgt ggaggacctg
      361 tggaaggcgt ggaagtcatc agaagtgtac aactggactg tggatgaggt gatacagtgg
      421 ctcattacgt atgtggagct gccacagtat gaggaaacct tccggaagtt gcagcttact
      481 ggccacgcca tgccaaggct agcagtaacc aacaccacca tgacagggac tgtactgaag
      541 atgacagatc ggagccacag gcagaagctg cagctgaagg ccctggacac agtgctgttt
      601 gggcctcctc tcttgactcg gcataatcac ctgaaggact tcatgctggt ggtgtctatc
      661 gttattggtg tgggtggctg ctggtttgcc tatatccaga accgttactc taaggagcac
      721 atgaagaaaa tgatgaagga tctggaaggg ggaggttcgg gggatccacc ggtcgccacc
      781 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      841 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      901 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      961 ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     1021 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1081 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1141 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1201 aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa gcagaagaac
     1261 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1321 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1381 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1441 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
//
