LOCUS       sequence-128190-         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKtheoRaj12-o11
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    36..57
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    287..305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    356..372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             419..430
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct tctagagaca
       61 gaaaagcccg cctttcggcg ggctttgccc agaatccctt cgagcaaatc ctagcgaggt
      121 gccgctagat tcgctcaaag gaacccttct tcctgcccgt ctggaatcca ggacacctgc
      181 caagggcctt tcggctggta tgtatttcgt cctatttggg actcatcagc tggatgtacg
      241 aaggcagcgc cgtgctcagt atctctatca ctgataggga tgtcaatctc tatcactgat
      301 agggagctag ccaattgatg catgctagca taaatgtgag cggataacat tgacattgtg
      361 agcggataac aagatactga gcaccctcgc ataatttcac ttcttcaatc ctcccgttaa
      421 agaggagaaa ttatgaatga ctagccgtaa aggcgaagag ctgttcactg gtgtcgtccc
      481 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
      541 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
      601 gccggtacct tgg
//
