LOCUS       sequence-128347-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for Firefly Luciferase Protein plant expression
            driven by the Nos promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB Pnos:Luciferase:Tnos (GB0112)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..117
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(218..397)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    493..515
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 gcgaggcatt tcgaagtact cagcgtaagt gatgtccacc tcgatatgtg catctgtaaa
       61 agcaattgtt ccaggaacca gggcgtatct cttcatagcc ttatgcagtt gctctccagc
      121 ggttccatct tccagcggat agaatggcgc cgggcctttc tttatgtttt tggcgtcttc
      181 cattgcctcg atcgagttga gagtgaatat gagactctaa ttggataccg aggggaattt
      241 atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg
      301 cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga
      361 aacccgcggc tcagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg
      421 cttgtcccgc gtcatcggcg ggggtcatac cgtgactccc ttaattctca tgtatctcct
      481 gagacgaatt cgttacacca caatatatcc tgccaagatc taattccggg gatcggaaat
      541 ccagaagccc gagaggttgc cgcctttcgg gctttttctt tttcaaaaaa aaaaatttat
      601 aaaacgatct gttgcggccg gccgccgggt tgtgggcaaa ggcgctcgac ggtgggcaac
      661 cgcttgcggt tgtccacggg cggagccggt gcgcgtagcg cattgtccac aagccaaggg
      721 cgaccaataa ttgatatata tattcataat tgaaaagcta attgaacata ctacttgctg
      781 taactacttg ccggagcgag gggtgtttgc aagctgttga tctgaaaggg ctattagcgt
      841 tctcacgtgc cttt
//
