LOCUS       sequence-128348-        5054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for Firefly Luciferase Protein plant expression
            driven by the Nos promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB Pnos:Luciferase:Tnos (GB0112)
FEATURES             Location/Qualifiers
     source          1..5054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             297..1949
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(363..381)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      1976..2228
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2263..2281)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2469..2493
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2584..3172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2664..2683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3343..4158)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4066..4085
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4150..4169
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4449..4884
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    5019..5041
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcaatgg
      301 aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg
      361 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
      421 ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc
      481 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
      541 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
      601 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc
      661 agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
      721 agctcccaat catccaaaaa attattatca tggattctaa aacggattac cagggatttc
      781 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
      841 tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta
      901 ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg
      961 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     1021 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     1081 tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga
     1141 ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg
     1201 acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg
     1261 aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc
     1321 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     1381 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     1441 tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg
     1501 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     1561 acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga
     1621 ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc
     1681 ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg
     1741 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     1801 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     1861 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     1921 ccaagaaggg cggaaagatc gccgtgtaat gagcttggaa tggatcttcg atcccgatcg
     1981 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgacgat
     2041 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     2101 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     2161 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcdcggtgt catctatgtt
     2221 actagatcgg gaattgccaa gctaattctt gaagacgaaa gggcctcgtg atacgcctat
     2281 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2341 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2401 tcatgagaca ataaccctga taaatgcttc aataatggga ccgactcgcg ctgtcatgag
     2461 acgaattctg acaggatata ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga
     2521 tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2581 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2641 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2701 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2761 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2821 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2881 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2941 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3001 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3061 taccttcgga agaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3121 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3181 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3241 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3301 taaatcaatc taaagtatat atgtgtaaca ttggtctagt gattagaaaa actcatcgag
     3361 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     3421 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     3481 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     3541 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     3601 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     3661 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa
     3721 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     3781 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     3841 tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     3901 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     3961 tgtaacaaca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     4021 cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     4081 atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc
     4141 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     4201 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac
     4261 gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc
     4321 cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta
     4381 caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca
     4441 ggcgatcccc atccaacagc ccgccgtcga gcgggctttt ttatccccgg aagcctgtgg
     4501 atagagggta gttatccacg tgaaaccgct aatgccccgc aaagccttga ttcacggggc
     4561 tttccggccc gctccaaaaa ctatccacgt gaaatcgcta atcagggtac gtgaaatcgc
     4621 taatcggagt acgtgaaatc gctaataagg tcacgtgaaa tcgctaatca aaaaggcacg
     4681 tgagaacgct aatagccctt tcagatcaac agcttgcaaa cacccctcgc tccggcaagt
     4741 agttacagca agtagtatgt tcaattagct tttcaattat gaatatatat atcaattatt
     4801 ggtcgccctt ggcttgtgga caatgcgcta cgcgcaccgg ctccgcccgt ggacaaccgc
     4861 aagcggttgc ccaccgtcga gcgccagcgc ctttgcccac aacccggcgg ccggccgcaa
     4921 cagatcgttt tataaatttt tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct
     4981 cgggcttctg gatttccgat ccccggaatt agagatcttg gcaggatata ttgtggtgta
     5041 acgaattcgt ctca
//
