LOCUS       sequence-128460-        1119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses suppression tRNA and mCherry inserted with a Npu DnaE 
            intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Ren W, Ji A, Ai HW
  TITLE     Light activation of protein splicing with a photocaged fast intein.
  JOURNAL   J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. 
            Epub 2015 Feb 5.
  PUBMED    25647354
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja508597d";
            journalName: "J Am Chem Soc"; date: "2015-02-18- 18"; volume: "137";
            issue: "6"; pages: "2155-8"
COMMENT     Sequence Label: pMAH2-intein/mCherry
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa ttgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagtagt taagctatga aacggaaata ttgacagtag aatatggatt attaccgatt
      481 ggtaaaattg tagaaaagcg catcgaatgt actgtttata gcgttgataa taatggaaat
      541 atttatacac aacctgtagc acaatggcac gatcgcggag aacaagaggt gtttgagtat
      601 tgtttggaag atggttcatt gattcgggca acaaaagacc ataagtttat gactgttgat
      661 ggtcaaatgt tgccaattga tgaaatattt gaacgtgaat tggatttgat gcgggttgat
      721 aatttgccga atatcaaaat agccacacgt aaatatttag gcaaacaaaa tgtctatgac
      781 attggagttg agcgcgacca taattttgca ctcaaaaatg gcttcatagc ttctaattgt
      841 atgaccatgg gctgggaggc ctccactgag cggatgtacc ccgaggacgg cgccctgaag
      901 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
      961 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt cgacatcaag
     1021 ttggacatcc tttcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     1081 ggccgccact ccaccggcgg catggacgag ctgtacaag
//
