LOCUS       sequence-128502-       10061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for FOXM1b expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Barger CJ, Zhang W, Hillman J, Stablewski AB, Higgins MJ, 
            Vanderhyden BC, Odunsi K, Karpf AR
  TITLE     Genetic determinants of FOXM1 overexpression in epithelial ovarian 
            cancer and functional contribution to cell cycle progression.
  JOURNAL   Oncotarget. 2015 Jul 16.
  PUBMED    26243836
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2015 Jul 16."
COMMENT     Sequence Label: pCW57.1-FOXM1b
FEATURES             Location/Qualifiers
     source          1..10061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             619..645
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             1933..1944
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(2934..2958)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2976..2993
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3003..3513
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3432..3450
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3523..4119
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3523..3542)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4019..4039
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4120..4173
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4174..4791,4792..4797,4798..4833,4834..4836,4837..4872,
                     4873..4875,4876..4911,4912..4920)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4960..4980)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5378..5389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5594..5827
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5899..6020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5936..5955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5990..6009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6060..6195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6195..6266
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6267..6335,6336..7127)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6485..6504)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7298..7886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7787..7806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8040..8057
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8174..8195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8210..8215,8216..8233,8234..8240)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8248..8264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8253..8275
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8272..8288
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8272..8288
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8307..8327
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8309..8327
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8355..8581
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8582..8762
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8809..8934
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9427..9660
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9845..9889
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tcgggaattc gtcgactgga tccggtaccg
      601 aggagatctg ccaccatgta cccatacgat gttccagatt acgctgcgat cgccggcgcg
      661 ccgaaaacta gcccccgtcg gccactgatt ctcaaaagac ggaggctgcc ccttcctgtt
      721 caaaatgccc caagtgaaac atcagaggag gaacctaaga gatcccctgc ccaacaggag
      781 tctaatcaag cagaggcctc caaggaagtg gcagagtcca actcttgcaa gtttccagct
      841 gggatcaaga ttattaacca ccccaccatg cccaacacgc aagtagtggc catccccaac
      901 aatgctaata ttcacagcat catcacagca ctgactgcca agggaaaaga gagtggcagt
      961 agtgggccca acaaattcat cctcatcagc tgtgggggag ccccaactca gcctccagga
     1021 ctccggcctc aaacccaaac cagctatgat gccaaaagga cagaagtgac cctggagacc
     1081 ttgggaccaa aacctgcagc tagggatgtg aatcttccta gaccacctgg agccctttgc
     1141 gagcagaaac gggagacctg tgcagatggt gaggcagcag gctgcactat caacaatagc
     1201 ctatccaaca tccagtggct tcgaaagatg agttctgatg gactgggctc ccgcagcatc
     1261 aagcaagaga tggaggaaaa ggagaattgt cacctggagc agcgacaggt taaggttgag
     1321 gagccttcga gaccatcagc gtcctggcag aactctgtgt ctgagcggcc accctactct
     1381 tacatggcca tgatacaatt cgccatcaac agcactgaga ggaagcgcat gactttgaaa
     1441 gacatctata cgtggattga ggaccacttt ccctacttta agcacattgc caagccaggc
     1501 tggaagaact ccatccgcca caacctttcc ctgcacgaca tgtttgtccg ggagacgtct
     1561 gccaatggca aggtctcctt ctggaccatt caccccagtg ccaaccgcta cttgacattg
     1621 gaccaggtgt ttaagcagca gaaacgaccg aatccagagc tccgccggaa catgaccatc
     1681 aaaaccgaac tccccctggg cgcacggcgg aagatgaagc cactgctacc acgggtcagc
     1741 tcatacctgg tacctatcca gttcccggtg aaccagtcac tggtgttgca gccctcggtg
     1801 aaggtgccat tgcccctggc ggcttccctc atgagctcag agcttgcccg ccatagcaag
     1861 cgagtccgca ttgcccccaa ggtgctgcta gctgaggagg ggatagctcc tctttcttct
     1921 gcaggaccag ggaaagagga gaaactcctg tttggagaag ggttttctcc tttgcttcca
     1981 gttcagacta tcaaggagga agaaatccag cctggggagg aaatgccaca cttagcgaga
     2041 cccatcaaag tggagagccc tcccttggaa gagtggccct ccccggcccc atctttcaaa
     2101 gaggaatcat ctcactcctg ggaggattcg tcccaatctc ccaccccaag acccaagaag
     2161 tcctacagtg ggcttaggtc cccaacccgg tgtgtctcgg aaatgcttgt gattcaacac
     2221 agggagagga gggagaggag ccggtctcgg aggaaacagc atctactgcc tccctgtgtg
     2281 gatgagccgg agctgctctt ctcagagggg cccagtactt cccgctgggc cgcagagctc
     2341 ccgttcccag cagactcctc tgaccctgcc tcccagctca gctactccca ggaagtggga
     2401 ggacctttta agacacccat taaggaaacg ctgcccatct cctccacccc gagcaaatct
     2461 gtcctcccca gaacccctga atcctggagg ctcacgcccc cagccaaagt agggggactg
     2521 gatttcagcc cagtacaaac cccccagggt gcctctgacc ccttgcctga ccccctgggg
     2581 ctgatggatc tcagcaccac tcccttgcaa agtgctcccc cccttgaatc accgcaaagg
     2641 ctcctcagtt cagaaccctt agacctcatc tccgtcccct ttggcaactc ttctccctca
     2701 gatatagacg tccccaagcc aggctccccg gagccacagg tttctggcct tgcagccaat
     2761 cgttctctga cagaaggcct ggtcctggac acaatgaatg acagcctcag caagatcctg
     2821 ctggacatca gctttcctgg cctggacgag gacccactgg gccctgacaa catcaactgg
     2881 tcccagttta ttcctgagct acagtagagc ccctcgaggt ttaaacggcc ggcacccagc
     2941 tttcttgtac aaagtggttt agtaatgaac cggtccacca ccaccaccac cactaaggat
     3001 ccggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     3061 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     3121 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     3181 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     3241 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     3301 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     3361 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     3421 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     3481 ctcgttgacc gaatcaccga cctctctccc cagcaattca ccatgaccga gtacaagccc
     3541 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     3601 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     3661 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3721 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3781 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3841 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3901 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3961 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     4021 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     4081 ggaccgcgca cctggtgcat gacccgcaag cccggtgccg aaggtagagg ttctctcctc
     4141 acttgtggtg atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc
     4201 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     4261 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     4321 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     4381 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     4441 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     4501 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     4561 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     4621 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     4681 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     4741 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     4801 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     4861 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     4921 ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg
     4981 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5041 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5101 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5161 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5221 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5281 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     5341 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5401 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5461 gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta
     5521 gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5581 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5641 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5701 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5761 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5821 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5881 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6001 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     6061 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6121 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6181 gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6241 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6301 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6361 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6421 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6481 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6541 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6601 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6661 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6721 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6781 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6841 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6901 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6961 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7021 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7081 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7141 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7201 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7261 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7321 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7381 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7441 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7501 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7561 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7621 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7681 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7741 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7801 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7861 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7921 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7981 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8041 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8101 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8161 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8221 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8281 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     8341 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     8401 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8461 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     8521 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     8581 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8641 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8701 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8761 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8821 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8881 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8941 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     9001 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     9061 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     9121 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     9181 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     9241 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     9301 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     9361 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9421 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9481 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9541 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9601 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9661 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9721 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9781 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9841 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9901 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9961 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    10021 accattatcg tttcagaccc acctcccaac cccgagggga c
//
