LOCUS       sequence-128547-         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated
            STIM1 protein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Kyung T, Lee S, Kim JE, Cho T, Park H, Jeong YM, Kim D, Shin A, Kim 
            S, Baek J, Kim J, Kim NY, Woo D, Chae S, Kim CH, Shin HS, Han YM, 
            Kim D, Heo WD
  TITLE     Optogenetic control of endogenous Ca channels in vivo.
  JOURNAL   Nat Biotechnol. 2015 Sep 14. doi: 10.1038/nbt.3350.
  PUBMED    26368050
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Sep 14. doi: 10.1038/nbt.3350."
COMMENT     Sequence Label: pCMV-OptoSTIM1
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(550..567)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattcaaggc atggagacag ggaaggacag ggtacccact cgtggatgct ggcatgagag
       61 agctctgggc tacaggctgg atgcacaacc gcatccgggt aatcgtgtcc tcatttgctg
      121 tcaagtttct gctcctgcct tggaaatggg gaatgaagta cttttgggat acccttctcg
      181 acgccgactt ggagtgtgac attctgggat ggcaatatat tagcgggtca attcctgacg
      241 gccatgagtt ggacaggttg gacaatccgg ccttgcaggg agctaagtat gatcccgaag
      301 gagagtatat tcgacagtgg ctccccgagc tggcccgact tcctacggag tggattcacc
      361 atccttggga cgcaccactg acagtgctca aggcaagcgg ggtggagctg ggcaccaatt
      421 acgctaagcc tatagttgat atagatacag cacgcgagct gctggctaaa gcgatctctc
      481 gcactcggga ggcgcanatt atgatcggtg ctgccgtgta cagtggggga ggcggagggg
      541 gtggttcagg tggtggtggt ggtggtgg
//
