LOCUS       sequence-128562-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for DsRed plant expression driven by the 35S 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 35S:DsRed:Tnos (GB0361)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..763
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(266..286)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     678..701
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tttggagagg actccggtat ttttacaaca attaccacaa caaaacaaac aacaaacaac
       61 attacaattt actattctag tcgaaatggc ctcctccgag aacgtcatca ccgagttcat
      121 gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga
      181 gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga ccaagggcgg
      241 ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta
      301 cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa
      361 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc aggactcctc
      421 cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga
      481 cggccccgtg atgcagaaga agacgatggg ctgggaggcc tccaccgagc gcctgtaccc
      541 ccgcgacggc gtgctgaagg gcgagacaca caaggccctg aagctgaagg acggcggcca
      601 ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta
      661 ctactacgtg gacgccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
      721 gcagtacgag cgcaccgagg gccgccacca cctgttcctg taggcttgga atggatcttc
      781 gatcccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccgg
//
