LOCUS       sequence-128803-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses Syt1-SNARE37aa-linker complex with pDC184.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Zhou Q, Lai Y, Bacaj T, Zhao M, Lyubimov AY, Uervirojnangkoorn M, 
            Zeldin OB, Brewster AS, Sauter NK, Cohen AE, Soltis SM, Alonso-Mori 
            R, Chollet M, Lemke HT, Pfuetzner RA, Choi UB, Weis WI, Diao J, 
            Sudhof TC, Brunger AT
  TITLE     Architecture of the synaptotagmin-SNARE machinery for neuronal 
            exocytosis.
  JOURNAL   Nature. 2015 Sep 3;525(7567):62-7. doi: 10.1038/nature14975. Epub 
            2015 Aug 17.
  PUBMED    26280336
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14975"; journalName: "Nature"; date: "2015-09-3- 3"; 
            volume: "525"; issue: "7567"; pages: "62-7"
COMMENT     Sequence Label: p045
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatgga gaaactggga aagctccaat
      181 attcactgga ctatgacttc cagaataacc agctgttggt gggaatcatc caggctgctg
      241 aactgcccgc cctggacatg gggggtacat ccgatccata cgtcaaagtc ttcctgctgc
      301 ctgacaaaaa gaagaaattt gagactaaag tccaccggaa aaccctcaat ccagtcttca
      361 atgaacaatt tactttcaag gtaccctact cggaattagg tggcaaaacc ctggtgatgg
      421 ctgtgtatga ctttgatcgc ttctccaagc acgacatcat cggagagttc aaagttccta
      481 tgaacaccgt ggattttggc catgtgaccg aggagtggcg cgatctccag agcgctgaga
      541 aagaagagca agagaaactg ggtgacatct gcttctccct ccgctacgtc cctactgccg
      601 gcaaactgac tgttgtcatt ctggaagcca agaacctgaa gaagatggat gtgggtggct
      661 tatctgatcc ctacgtgaag attcacctga tgcagaacgg taagaggctg aagaagaaaa
      721 agacgacgat taagaagaac acactcaacc cctactacaa cgagtccttc agctttgaag
      781 ttccgttcga gcaaatccag aaagtgcaag tggtggtaac tgttttggac tatgacaaga
      841 ttggcaagaa cgacgccatc ggcaaagtct tcgttggtta caacagcact ggggcggagc
      901 tgcgacactg gtcagacatg ctggccaacc cccggcgacc catcgcacag tggcacactc
      961 tgcaggtaga ggaggaggtt gatgccatgc tggctgtcaa gaagaacctg tcgtcggata
     1021 gctccttgtc ctctccgtcc gctttgaata gcctgtcctc tccttcggcc ctgaactcga
     1081 cggcaagcaa ta
//
