LOCUS       sequence-128805-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses Syt1-SNARE37aa-linker complex with pDC184.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Zhou Q, Lai Y, Bacaj T, Zhao M, Lyubimov AY, Uervirojnangkoorn M, 
            Zeldin OB, Brewster AS, Sauter NK, Cohen AE, Soltis SM, Alonso-Mori 
            R, Chollet M, Lemke HT, Pfuetzner RA, Choi UB, Weis WI, Diao J, 
            Sudhof TC, Brunger AT
  TITLE     Architecture of the synaptotagmin-SNARE machinery for neuronal 
            exocytosis.
  JOURNAL   Nature. 2015 Sep 3;525(7567):62-7. doi: 10.1038/nature14975. Epub 
            2015 Aug 17.
  PUBMED    26280336
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14975"; journalName: "Nature"; date: "2015-09-3- 3"; 
            volume: "525"; issue: "7567"; pages: "62-7"
COMMENT     Sequence Label: p045
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(28..75)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     71..89
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(127..171)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    460..484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(484..503)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(485..503)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
       61 aaggggttat gctagttatt gctcagcggt ggcagcagcc taggttaatt aagctgcgct
      121 agtagacgag tccatgtgct ggcgttcaaa tttcgcagca gcggtttctt taccagactc
      181 gagttatttg cctaaatctt ttaaattttt ctcggcctcc ttcatgtctt ggttgatatg
      241 gttcatgcct tcttcgacac gatcgagttg ttcgccttgc tcatccaaca taaccaaagt
      301 cctgatgcca gcatctttac tctcttcaac cagctgcagc atgcgacggg tgctttccag
      361 ggactcatca gccagctggt cagccctcct ctgcatctcc tccagctcat tgcgcatatg
      421 tatatctcct tcttatactt aactaatata ctaagatggg gaattgttat ccgctcacaa
      481 ttcccctata gtgagtcgta ttaatttcga ttatgcggcc gtgtacaata cgattacttt
      541 ctgttcgact taagcattat gcggccgcaa gcttttagcc cagcatcttt gttgcacgtt
      601 ggttggcttc atcaattctg gttttgttgg aatcagcctt ctccatgatc ctgtcgatct
      661 ggcgattctg ggtgtcaatc tcattgccca tgtctagtgc catatggcgg aggtttccga
      721 tgatgccgct cacctgctcc aggttctcat ccatctcatt ttcccgggcg ctcagcccct
      781 caattcccgg actattgctt gccgtcgagt tcagggccga aggagaggac aggctattca
      841 aagcggacgg agaggacaag gagctatccg acgacaggtt cttcttgaca gccagcatgg
      901 catcaacctc ctcctctacc tgcagagtgt gccactgtgc gatgggtcgc cgggggttgg
      961 ccagcatgtc tgac
//
