LOCUS       sequence-128851-       12220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB229
FEATURES             Location/Qualifiers
     source          1..12220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2071..3501
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     terminator      3561..3813
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3847..3863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3847..3863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3860..3882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3871..3887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3895..3901,3902..3919,3920..3925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3940..3961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4046..4218
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4275..4808)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4871..5547)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4914..4934)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5742..5766
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6287..7078
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7324..7912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7813..7832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8066..8083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8098..8238
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8164..8183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8324..8346
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8582..8776
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8842..9915)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10344..10973)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtgcaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagcg ctggaggctc cggtgggagc atggtgagca agggcgagga ggtcatcaaa
     2101 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     2161 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2221 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     2281 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     2341 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     2401 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     2461 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     2521 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     2581 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     2641 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     2701 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc
     2761 ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc
     2821 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     2881 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     2941 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     3001 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     3061 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     3121 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     3181 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     3241 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     3301 aaggacggcg gccgctacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     3361 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     3421 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc
     3481 atggacgagc tgtacaagta agcttgcatg cctgcaggtc gactctagag gatccccggg
     3541 taccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     3601 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     3661 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     3721 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     3781 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta
     3841 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3901 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3961 aggctgaatt aggcgcgcct atttctgaat tcaacacatt gcggacgttt ttaatgtact
     4021 gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga
     4081 attagatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     4141 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     4201 cacattatta tggagaaact cgagcttgtc gatcgactct aggccgggaa gccgatctcg
     4261 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
     4321 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
     4381 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
     4441 gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta
     4501 cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg
     4561 tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag
     4621 tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga
     4681 gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata
     4741 cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg
     4801 cgtaccatgg tcgatcgaca gatctgcgaa agctcgagag agatagattt gtagagagag
     4861 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
     4921 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
     4981 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     5041 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     5101 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     5161 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     5221 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     5281 cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     5341 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     5401 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
     5461 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
     5521 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
     5581 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
     5641 tacgcaaacc gaattcagaa ataaattcag cctaattcgg cgttaattca gtacattaaa
     5701 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     5761 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     5821 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     5881 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     5941 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     6001 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     6061 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     6121 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     6181 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     6241 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     6301 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     6361 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     6421 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     6481 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     6541 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     6601 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     6661 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     6721 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     6781 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     6841 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     6901 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     6961 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     7021 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     7081 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     7141 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     7201 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     7261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7321 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7381 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7441 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7501 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7561 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7621 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7681 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7741 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7801 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7861 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7921 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7981 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8041 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     8101 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     8161 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     8221 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     8281 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     8341 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     8401 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
     8461 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     8521 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     8581 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     8641 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     8701 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     8761 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
     8821 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     8881 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     8941 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     9001 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     9061 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     9121 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     9181 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     9241 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     9301 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     9361 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     9421 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     9481 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     9541 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     9601 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     9661 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     9721 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     9781 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     9841 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     9901 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     9961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
    10021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
    10081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
    10141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
    10201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
    10261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
    10321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
    10381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
    10441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
    10501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
    10561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
    10621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    10681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    10741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    10801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    10861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    10921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    10981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    11041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    11101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    11161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    11221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    11281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    11341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    11401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    11461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    11521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    11581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    11641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    11701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    11761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    11821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    11881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    11941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    12001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    12061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    12121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
    12181 tggttggcat gcacatacaa atggacgaac ggataaacct
//
