LOCUS       sequence-128895-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Histone H3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG333
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(579..623)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(636..656)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
       61 ctacaaatgt ggtatggctg attatgatca gttatctaga tccggtggat cccgggcccg
      121 cggtaccgtc gactgcagaa ttcttatgcc ctttccccac ggatgcggcg agcgagctgg
      181 atgtctttgg gcataatagt cactcgctta gcatggatgg cgcaaaggtt tgtgtcctca
      241 aagagcccta ccaagtaggc ctcacaagcc tcctgcagcg ccatcaccgc agagctctgg
      301 aagcgaagat cggtcttgaa gtcttgggcg atttctcgca ccaggcgctg gaacggcagc
      361 ttccgaatca gcaactcggt cgacttttgg tagcggcgga tctcgcgcag agccacagtg
      421 cccgggcggt aacggtgagg ctttttcacg ccgccggtag ccggcgcgct cttgcgagca
      481 gccttggtag ccagctgctt gcgtggcgct ttaccgccgg tggatttccg agctgtttgc
      541 ttcgtacgag ccatctcgag actagtacct ccacctccgc tgtccatgtg ctggcgttcg
      601 aatttagcag cagcggtttc tttgaatttg gcggcgccct gaaaataaag attctctcta
      661 gagcggccgc caccggtgga gctccaccga tcccctccgg acttgtacag ctcgtccatg
      721 ccgagagtga tcc
//
