LOCUS       sequence-128924-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of guide RNA with fluorescent GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, 
            Wilcox S, Tai L, Strasser A, Herold MJ
  TITLE     An inducible lentiviral guide RNA platform enables the 
            identification of tumor-essential genes and tumor-promoting 
            mutations in vivo.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 
            10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
  PUBMED    25732831
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.02.002"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1422-32"
COMMENT     Sequence Label: FgH1tUTG
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    126..243
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     434..453
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    498..516
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        538..613
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
      121 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      181 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      241 tttcgggttt attacaggga cagcagagat ccagtttggt taattaacca tggaattcga
      301 acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca
      361 cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg
      421 caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga
      481 tttgggaatc ttataagtcc ctatcagtga tagagattcc cggagacgga cgtctcggtt
      541 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
      601 accgagtcgg tgcttttttt ttaattaacc cgtgtcggct ccagatctgg cctccgcgcc
      661 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcag
//
