LOCUS       sequence-129072-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of mCherry with FKBP12 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: pmCherry-FKBP12-C1
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    129..185
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(join(228..548,549..551))
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
      121 gatccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcgaagc ttgagctcga
      181 gatctccggt accctgctcc tcctgctccc gctgctgcgc ctcgagcttc cagttttaga
      241 agctccacat cgaagacgag agtggcatgt ggtgggatga tgcctgggtg cccagtggca
      301 ccataggcat aatctggaga tatagtcagt ttggctctct gacccacact catctgggca
      361 accccttctt cccagcctcg gatcacctcc tgcttgccta gcataaactt aaagggcttg
      421 tttctgtccc gggaggaatc aaatttcttt ccatcttcaa gcatcccggt gtagtgcacc
      481 acgcaggtct ggccgcgctt ggggaaggtg cgcccgtctc ctggggagat ggtttccacc
      541 tgcactccca tcgccgctct gtacagctcg tccatgccgc cggtggagtg gcggccctcg
      601 gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg
      661 atgttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg
      721 acctcagcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc
      781 a
//
